Abstract
IN VITRO ANTI-INFLAMMATORY ACTIVITY OF ETHANOLIC EXTRACT OF BRASSICA RAPA SUBSP.RAPA

Aparna Lakshmi I.*, Prakashita Goud, Dr. H. Doddayya, Dr. T. Shivaraj Gouda, Prem Kattimani and Rushikesh Tate

ABSTRACT

Aim: To investigate the anti-inflammatory activity of ethanolic extract of Brassica rapa leaves. Materials and Methods: The anti-inflammatory activity of Ethanolic Extract of Brassica rapa leaves (EEBR) was evaluated by using in vitro based assays: Human Red Blood Cell (HRBC) membrane stabilization assay, Heat Induced Hemolysis and Protein Denaturation Inhibition. Results: The results showed that the percentage stabilization of EEBR on HRBC membrane under hypotonicity was within the range of 19.32 to 66.21% and EEBR has exhibited a significant % inhibition on heat induced hemolysis (21.3 to 68.7%) when compared to that of standard. The percentage inhibition on protein denaturation of EEBR was within the range of 34.85 to 72.80% and EEBR at a dose of 1500µg/ml has exhibited significantly higher inhibition level. Conclusion: The results revealed that EEBR possess anti- inflammatory properties and could have a potential therapeutic effect on disease process causing destabilization of biological membrane.

Keywords: Brassica rapa, Protein denaturation, Hemolysis, Membrane stabilization.


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