EVALUATION OF EFFECT ETHANOLIC EXTRACT OF ANNONA SQUAMOSA LEAF ON GABA MEDIATED ANXIETY
Hitesh Kumar Gupta*, Kehar Singh Dhaker and Vishal Shrivasatava
ABSTRACT
The objective of the present investigation was to evaluate the evaluate the anxiolytic action of ethanolic extract of Annona squamosa leaf using light/dark box model and elevated plus maze model and to establish the possible involvement of GABA in the action of the extract. Extraction was carried out using ethanol after defatting the dried leaf powder with n-hexane. The total phenolic content in the leaf extract would be determined quantitatively using Folin-Ciocalteu reagent method, using gallic acid as the reference standard. Light/dark box model as well as elevated plus maze model were used to determine the anxiolytic action of the extract at doses of 200 and 400 mg/Kg administered intraperitoneally in mice. Co administration of flumazenil was used to study the involvement of GABA in the anxiolytic activity. The ethanolic extract was dark green in color and sticky and obtained in 16.1% yield. The total phenolic content of the ethanolic extract of Annona squamosa leaf was found to be 25.16 ± 0.175 GAE/mg. The extract at a dose of 400 mg/kg was able to significantly increase the time spent by the mouse in the lit box. The extract at 200 mg/kg dose did not exhibit a significant improvement in open arm entries by the mouse whereas the extract at 400 mg/kg dose exhibited a significant result compared to control (p<0.001) in Two way ANOVA. The number of movements in open arm on administration of ethanolic extract of Annona squamosa leaf (400 mg/kg) was found to be 5.16 ± 0.048 as compared to a negligible movement of 1.5 ± 0.047 on the normal saline treated mice.
Keywords: GABA, anxiety, elevated plus maze, light/dark box test, Annona squamosal.
[Full Text Article]
[Download Certificate]
Impact Factor : 8.181 
