European Journal of Biomedical and Pharmaceutical Sciences

EXTRACTION AND IDENTIFICATION OF ALKALOID-VASICINE FROM ADHATODA VASICA BY USING THIN LAYER CHROMATOGRAPHY

*Pushpalata P. Sherekar, Tejas Talekar, Yashashree Ugave, Inamulhasan Tamboli, Aarti Tate and Astha Tambe

ABSTRACT

Adhatoda vasica: Due to its expectorant properties, nees, also known as vasaka, is a plant that is widely used in indigenous systems of medicine. In addition, the herb can be used to treat leprosy, asthma, cough, and other skin conditions. Vasicine is the main phytochemical present in the plant and is a quinazoline alkaloid. Numerous alkaloids, including vasicinone, deoxy vasicine, vasicinol, etc., are also present in the vasaka plant in addition to vasicine. The purpose of the effort is to offer a quick and affordable way for getting vasicine out of Adhatoda vasica leaves. In this study, a modified version of the conventional acid-base extraction procedure was used to isolate vasicine from the leaves of Adhatoda vasica. Thin Layer Chromatography was used to further purify the leaf-isolated alkaloid combination. By observing under UV light at 254 nm, standard vasicine from Natural Remedy was used to establish the presence of purified vasicine. The reference standard's Rf value was 0.55, whereas the purified vasicine's Rf value was 0.561 ± 0.039.

Keywords: Adhatoda vasica, vasicine, quinazoline alkaloid, acid-base extraction, thin layer chromatography.