European Journal of Biomedical and Pharmaceutical Sciences

ISOLATION, CHARACTERISATION, AND SCREENING OF GUT BACTERIA FOR ESSENTIAL PROBIOTIC CHARACTERISTICS

Nishi J. Patel*, Dr. Murtaza Hajoori and Dr. Piyush Desai

ABSTRACT

The human gastrointestinal system is home to a wide variety of microbes that collectively comprise a unique microbiota characteristic of every individual. Healthy microbiota are those that are normal and sustain and promote overall health and the absence of disease, particularly in the gastrointestinal system. The basis for probiotic therapy is the improvement of the native microbiota's unbalanced characteristics. Here we have identified 4 such bacteria isolated from the gut that can be used as potential probiotics. The bacteria have been screened for probiotic characteristics like resistance to pH, resistance to bile salts, resistance to phenol, Haemolytic test, DNase test, antibacterial test, and antifungal test. Various biochemical tests have characterized the isolates. Four isolates have been considered to have good probiotic properties on the basis of biochemical characterization two of the isolates are presumed to be Bacillus subtilis sp. and the other two belong to Lactobacilli sp.

Keywords: Gut bacteria, Probiotics, Gut modulation.