European Journal of Biomedical and Pharmaceutical Sciences

GENETIC CHARACTERIZATION OF AEROMONAS SP. BY SUBJECTING RAPD FINGERPRINT PATTERNS TO UPGMA CLUSTER METHOD ANALYSIS

Nidhi Saxena,* and Kiran Singh

ABSTRACT

Typing of bacterial pathogens for identifying them at strain level is important for diagnosis, treatment and epidemiological survey of bacterial infections. Aeromonas sp. are gram negative pathogenic bacteria of wide spectrum of organisms including humans. Different genetic factors like cytolytic enterotoxin gene AHCYTOEN, aerolysin gene, hemolysin gene have been attributed for its pathogenicity. Characterization of Aeromonads to the species level is difficult due to their phenotypic and genotypic heterogeneity. The aim of this was to use RAPDPCR fingerprint pattern as a molecular marker and computer assisted pattern analysis to characterize Aeromonas strains. Dendrogram constructed using RAPD-PCR fingerprint patterns demonstrated distinctive clusters at various similarity levels and was helpful in phylogenetic studies of Aeromonas sp.

Keywords: RAPD-PCR, Jaccord’s Coefficient, UPGMA, NTSYS pc Software, Aeromonas.